來自食用海藻的阿塞諾脂
arsenolipids from edible seaweed by lc-icp-ms and lc-esi-ms
conditions
column: ace 3 c18
dimensions: 150 x 4.6 mm
part number: ace-111-1546
mobile phase:
a: 0.1% formic acid in h2o
b: 0.1% formic acid in meoh
time(mins)
%b
0
0
20
100
45
100
flow rate: 1 ml/min
injection: 100 μl
temperature: 45 °c
detection: split ratio: 75% esi-ms: 25% icp-ms
thermo scientific element 2 icp-ms
mode: organic mode
medium resolution
thermo scientific orbitrap discovery
positive esi mode
spray voltage: 4.5 kv
capillary temperature: 320 °c
capillary voltage: 42 v
arsenic-containing hydrocarbon:
m/z 389 [m + h]+ for c21h46aso
arsenic-containing phospholipids:
m/z 959 [m + h]+ for c45h89aso14p (c16:0/c16:0)
m/z 987 [m + h]+ for c47h93aso14p (c18:0/c16:0)
m/z 1015 [m + h]+ for c49h97aso14p (c20:0/c16:0)