enzo life sciences的mito-id®membrane potential cytotoxicity kit利用陽(yáng)離子雙發(fā)射染料檢測(cè)線粒體膜電位（mmp）的波動(dòng)，該染料在細(xì)胞質(zhì)中以綠色熒光單體的形式存在，在線粒體中以橙色熒光j聚集體的形式聚集。具有低膜電位的線粒體將積累低濃度的染料并呈現(xiàn)綠色熒光，而更高度極化的線粒體將呈現(xiàn)橙紅色熒光。隨著線粒體功能受損加劇，細(xì)胞表現(xiàn)出從橙色熒光到綠色熒光的轉(zhuǎn)變。該試劑盒是一種獨(dú)-特的hts測(cè)定法，無(wú)需洗滌或去除培養(yǎng)基即可實(shí)時(shí)監(jiān)測(cè)線粒體膜電位。
產(chǎn)品特點(diǎn)
●靈敏度是jc-1熒光染料的10倍，并具有卓-越的水溶性
●具備光穩(wěn)定性的雙發(fā)射染料
●無(wú)需漂洗/換液步驟
●單獨(dú)的mito-id®檢測(cè)可用于檢測(cè)線粒體質(zhì)量
●可在較低的藥物/劑量濃度下檢測(cè)細(xì)胞毒性
●沒(méi)有使用jc-1染料時(shí)出現(xiàn)的溶劑偽影
●適用于高通量應(yīng)用
實(shí)驗(yàn)示例
圖1.檢測(cè)線粒體紊亂的靈敏度是jc-1的10倍。使用mito-id®膜電位染料（紅色）或jc-1（藍(lán)色）在經(jīng)cccp處理的hela細(xì)胞中評(píng)估線粒體膜電位（mmp）。使用傳統(tǒng)的熒光酶標(biāo)儀檢測(cè)，結(jié)果顯示mmp隨著cccp濃度的增加而減少，橙色熒光減少。染料的水溶性優(yōu)化和無(wú)需洗滌的實(shí)驗(yàn)方案最大限度減小了可變性，因此z因子（>0.9）高于使用jc-1.
圖2.在藥物篩選中實(shí)時(shí)檢測(cè)有絲分裂毒性。使用biotek synergy™ mx熒光酶標(biāo)儀對(duì)線粒體膜電位變化的時(shí)間進(jìn)程研究。hela細(xì)胞與mito-id®膜電位染料在室溫下孵育30分鐘（不去除血清或培養(yǎng)基）。添加魚藤酮分別達(dá)到1µm、3µm和9µm的濃度。橙色信號(hào)的減少證明染料對(duì)魚藤酮有反應(yīng)。
產(chǎn)品信息
產(chǎn)品貨號(hào)
enz-51019-kp002
產(chǎn)品名稱
mito-id®membrane potential cytotoxicity kit
規(guī)格
1 kit
短期保存
-20°c
長(zhǎng)期保存
-80°c
試劑盒組分
mito-id®mp detection reagent, 200 μl
cccp control, 100 μl
10x assay buffer 1: 2.5 ml
50x assay buffer 2: 0.5 ml
應(yīng)用
htsmicroplate
部分產(chǎn)品引用文獻(xiàn)
1. novel silver complexes based on phosphanes and ester derivatives of bis(pyrazol-1-yl)acetate ligands targeting trxr: new promising chemotherapeutic tools relevant to sclc management: m. pellei, et al.; int. j. mol. sci.24, 4091 (2023)
2. fundc1 regulates receptor-mediated mitophagy independently of the pink1/parkin-dependent pathway in rotenone-treated sh-sy5y cells: s.y. park, et al.; food chem. toxicol.137, 111163 (2020),application(s):fluorescence microscopy and microplate reader
3. inhibitory role of trip-br1/xiap in necroptosis under nutrient/serum starvation: z. sandag, et al.; mol. cells43, 236 (2020)
4. nad hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages: d. pajuelo, et al.; cell. microbiol.22, e13115 (2020),application(s):thp-1 macrophages; microplate reader
5. impaired autophagic and mitochondrial functions are partially restored by ert in gaucher and fabry diseases: m.m. ivanova, et al.; plos one14, e0210617 (2019),application(s):fluorescence microscopy
6. inhibitory role of ampactivated protein kinase in necroptosis of hct116 colon cancer cells with p53 null mutation under nutrient starvation: d.t. le, et al.; int. j. oncol.54, 702 (2019)
7. ros as a novel indicator to predict anticancer drug efficacy: t. zaidieh, et al.; bmc cancer19, 1224 (2019)
8. trem1/3 deficiency impairs tissue repair after acute kidney injury and mitochondrial metabolic flexibility in tubular epithelial cells: a. tammaro, et al.; front. immunol.10, 1469 (2019),application(s):microplate reader
9. anti-cancerous effect of cis-khellactone from angelica amurensis through the induction of three programmed cell deaths: s. jung, et al.; oncotarget9, 16744 (2018),application(s):microplate reader
10. blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions: v. marek, et al.; free radic. biol. med.126, 27 (2018),application(s):microplate reader
11. cgas drives noncanonical-inflammasome activation in age-related macular degeneration: n. kerur, et al.; nat. med.24, 50 (2018)
12. cytotoxicity of propofol in human induced pluripotent stem cell-derived cardiomyocytes: k. kido, et al.; j. anesth.32, 120 (2018),application(s):microplate reader
13. development of novel amino-quinoline-5, 8-dione derivatives as nad (p) h: quinone oxidoreductase 1 (nqo1) inhibitors with potent antiproliferative activities: y. ling, et al.; eur. j. med. chem.154, 199 (2018)
14. inhibition of apoptosis using exosomes in chinese hamster ovary cell culture: s. han, et al.; biotechnol. bioeng.115, 1331 (2018)
15. light action spectrum on oxidative stress and mitochondrial damage in a2e-loaded retinal pigment epithelium cells: m. marie, et al.; cell death disc.9, 287 (2018)
詳情請(qǐng)聯(lián)系enzo life sciences金牌代理——欣博盛生物